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. 2020 Feb 20;10:3069. doi: 10.1038/s41598-020-59430-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Skeletal muscle ablation of Pit1 and Pit2 results in a mild renal Pi leak at P10. P10 smPit1^−/−; smPit2^−/− mice were hypophosphatemic (A) and hyperphosphaturic (B) in an FGF23-independent fashion (C). Blood glucose (D) and BUN were within normal limits (E). Means ± SEM, n = 5–10, ^***p = 0.0002, ^**p = 0.002, ^*p = 0.03 vs. WT.