Fig. 5.

Immunoblotting of Rg3(S)-induced proteins in human dermal fibroblasts (HDFs). (A) Young (passage #6) and old (passage #17) HDFs were pretreated with 10 μM Rg3(S) for 24 h. (B) Old HDFs were incubated with a high dose of Rg3(S) (80 μM) for 3 to 9 days. At each time point, cells were collected, and the expression levels of Hsp60, PRDX, PRDX2, and PRDX3 were analyzed by immunoblotting. To compare relative expression levels, β-actin was used as loading control. Quantification of protein expression levels was performed using ImageJ. Statistical significance: ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.

PRDX, peroxiredoxin.