Fig. 3.

BST204 inhibits the adipogenic commitment from mesenchymal stem cells. (A) Schematic representation of 10T1/2 cell differentiation into adipocytes. The protein and mRNA samples used in Fig. 3 were extracted from 10T1/2 cells at d0. (B) Immunoblot analysis of 10T1/2 cells treated with or without BST204 (50 μg/ml) for 4 days in the presence or absence of BMP4, which is inducer of adipogenic commitment. (C) The mRNA levels of the PPARγ and Cebpα genes in 10T1/2 cells treated with or without BST204 (50 μg/ml) for 4 days in the presence or absence of BMP4. Data represent mean ± SEM for n = 3. *P < 0.05, **P < 0.01, ***P < 0.001.

MSC, mesenchymal stem cell; SEM, standard error of the mean.