Table 3.
Details for the dCAPS markers developed to authenticate Panax species
| Marker ID | Primer sequence (5′-3′) | Location | Tm (°C) | PCR product size (bp) | Digestion enzyme | Target SNP |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Pg | Pq | Pn | Pj | Pv | Ps | Pt | ||||||
| Pgdm1 | GTTTAAATTATTCCGGTGGATTCTT | rpl20 | 59.2 | 170 | Cla1 | A | G | G | G | G | G | G |
| GTAGCCTATAGTTATAGTAGATTAATCGA | 63.4 | |||||||||||
| Pgdm2 | GTCCGCTTGTCTAGGACTCG | ndhK | 62.5 | 177 | Cla1 | A | G | G | G | G | G | G |
| CAAAATTCAGTTATTTCAACTACATCAAT | 60.5 | |||||||||||
| Pgdm3 | ATCCAACCGACCAATTAATTCTTTA | rps15 | 59.2 | 219 | Sma1 | C | T | T | T | T | T | T |
| TTGAAAGAGGAAAACAAAGACACCC | 62.5 | |||||||||||
| Pqdm4 | TATGACCGTCCCTCATCGGTTGTCG | rpoC1 | 69.1 | 212 | Sal1 | G | A | G | G | G | G | G |
| CATTCAGATAGATGGGGGTAAACTA | 62.5 | |||||||||||
| Pqdm5 | CTCGTAAACCACCTAAAAAGGAAT | ndhA | 60.1 | 206 | Cla1 | C | T | C | C | C | C | C |
| TCGTTTATTCAGTATCGGACCATCG | 64.2 | |||||||||||
| Pqdm6 | TTCCGGCTGTTAAAATTAGGTCAGC | ndhK | 63 | 167 | Alu1 | T | C | T | T | T | T | T |
| TCTTTCAAATTGGTCAAGACTCTCT | 60.9 | |||||||||||
| Pndm7 | CCTATTTACACAAATACCCCGTCGA | rpoC1 | 64.2 | 223 | Sal1 | T | T | C | T | T | T | T |
| ATTAGTTCGTAAAGGATTCAATGCAG | 61.6 | |||||||||||
| Pndm8 | TTCATTTGATCTTGATCCTTGTG | rpoC2 | 57.5 | 216 | HindIII | A | A | G | A | A | A | A |
| TCCACTTTGAATTTTAAAGAGAAGCT | 60.0 | |||||||||||
| Pndm9 | ATCGACAGGAATTAGCTTATCGAC | ndhK | 61.8 | 238 | Cla1 | G | G | A | G | G | G | G |
| ACGATTCGACTTTGATCGTTATCGA | 62.5 | |||||||||||
| Pjdm10 | TGGATATCTCCAGAAAATATTTTAAGTAC | rpoC2 | 62.0 | 250 | Sal1 | A | A | A | T | G | A | A |
| AGGATTTGATTGAGTATCGAGGAG | 61.8 | |||||||||||
| Pjdm11 | AGTCCGACATTTATTCCTTCAGAC | rpoB | 61.8 | 172 | Rsa1 | T | T | T | C | T | T | T |
| GTTTTGGATCGAACTAATCCATTGGT | 63.2 | |||||||||||
| Pvdm12 | TGCGCGAATCTCAGCAATCACTAG | rpoC2 | 65.3 | 195 | Spe1 | T | T | T | T | C | T | T |
| AAATTCAATGAGGATTTGGTTCAT | 56.7 | |||||||||||
| Pvdm13 | CATAAGGTAAATACTGTATAATTGATCG | ndhH | 61.3 | 170 | Cla1 | G | G | G | G | A | G | G |
| TATGATAGTCAATCTGGGTCCTCA | 61.8 | |||||||||||
| Psdm14 | AACCTTCTTTGGATTTGCCCAAGCT | psbB | 64.2 | 166 | HindIII | C | C | C | C | C | T | C |
| CACGCTGGATTTACAGATTGTACT | 61.8 | |||||||||||
| Psdm15 | GAAGCCACAAAGGACTATCTAAATG | rpoC1 | 62.5 | 180 | EcoR1 | G | G | G | G | G | A | G |
| GTCGGGGTATTTGTGTAAATAGGT | 61.8 | |||||||||||
| Psdm16 | TAAGCTTCCTTCCTATTAATCTGGGAATT | rpoB | 64.8 | 179 | EcoR1 | C | C | C | C | C | T | C |
| CATATTAGAGCTCGCCAGGAAGTA | 63.5 | |||||||||||
| Ptdm17 | TATGTACGGAATAGAAAGATTCCAAGC | ndhA | 63.7 | 187 | Alu1 | C | C | C | C | C | C | T |
| CGAGTGTGAGAGATTACCTTTTGA | 61.8 | |||||||||||
| Ptdm18 | CGCTCTATTTAGCAATACGGGATA | rpoC1 | 61.8 | 162 | EcoRV | C | C | C | C | C | C | T |
| GCAATAGAGCTTTTCCAGACATTT | 60.1 | |||||||||||
dCAPS, derived cleaved amplified polymorphic sequence; SNP, single nucleotide polymorphism; PCR, polymerase chain reaction; Pg, Panax ginseng; Pq, P. quinquefolius; Pn, P. notoginseng; Pj, P. japonicus; Pv, P. vietnamensis; Ps, P. stipuleanatus; Pt, P. trifolius.