Table 6.
Primer for qRT-PCR.
| Unigene | Primer sequences (5ʹ-3ʹ) | Annealing temperature (°C) | Product length (bp) |
|---|---|---|---|
| c20892_g1 | F: GTGCCTTTTATCTTCGCCTACT | 57.8 | 142 |
| R: GGAACGCCTCAGAAAGATTG | 57.3 | ||
| c50240_g6 | F: AGATTTCTACTACTTCAGCAGCCG | 59.6 | 116 |
| R: GGTTTTGACGCAACACCTTC | 57.9 | ||
| c53242_g1 | F: TTCGCAGACTTTACAACGGAC | 58.1 | 134 |
| R: CTCAACCCTCTGATGAAGTCGT | 58.3 | ||
| c50620_g1 | F: CAACAAAATGGGAGCAATAGG | 57.7 | 75 |
| R: TCTACTTTCAGAATAGCGATGGG | 58.5 | ||
| c55114_g3 | F: ATGCTGTGGTCTATGGTGTTGA | 57.8 | 234 |
| R: CTTTATTTGGGTCGTAGCCTTC | 58.1 | ||
| c49424_g3 | F: GTGCCAAGGTCAAGTCAGTGT | 57.2 | 129 |
| R: CGGTCATCACAGCCTTCAAC | 58.5 | ||
| c55056_g1 | F: GGTTTCCACAACTACCACCACA | 59.7 | 142 |
| R: CGTCGGAGACCGTTTTCAA | 59.2 | ||
| c48666_g1 | F: TCCTTACGGTATGTTTGGCG | 58.8 | 230 |
| R: TGTCTGCTTTCTGGACTGGG | 58.2 | ||
| c56307_g1 | F: TACGACGCAAAGACTGAGGAA | 58.5 | 158 |
| R: TGAACTATGAAGGGGTGGATG | 57.3 | ||
| c54537_g1 | F: GAAGCAGTATTTCCCCGATTT | 58.0 | 229 |
| R: TTGGACAGAGTACATGCGTTTG | 58.8 | ||
| c52770_g1 | F: TAACGGGTTTCTTGGCTTTG | 58.2 | 248 |
| R: GTTCTGGTCCATCCTTTCTCAC | 57.7 | ||
| c45463_g2 | F: GTTTTGGCGGTGGTGACTT | 58.0 | 128 |
| R: GGCTTCTTGGTTTACATCCCT | 57.8 | ||
| c54918_g4 | F: GACTGATTGACGAGCCACTACTT | 58.0 | 93 |
| R: TCTTCTCCGCCTTGGTCTTA | 57.5 | ||
| Of- actin | F: ATCATCACCAACTGGGACGA | 58.0 | 139 |