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. 2020 Feb 5;23(3):100887. doi: 10.1016/j.isci.2020.100887

Figure 6.

Figure 6

HP Activation in C. elegans Ameliorates polyQ Toxicity in a Cell-Autonomous Manner

(A) Representative images of tissue-specific gfat-1 OE strains at late L4 larval stage with cloning schemes, muscle-specific gfat-1 OE (myo-3P::CFP::gfat-1, left panel) and pan-neuronal gfat-1 OE (rgef-1P::CFP::gfat-1, right panel).

(B) Representative images of polyQ aggregates in muscle tissue of myo-3P::CFP::gfat-1; unc-54P::Q35::YFP animals relative to controls at day 4 of adulthood. Higher magnification of indicated area in lower panel.

(C) Quantification of Q35::YFP aggregates at day 4 of adulthood from microscopy images. Mean + SD (n = 10 animals per condition) ***p < 0.001 (ANOVA).

(D) Motility assay of gfat-1 OE strains in unc-54P::Q35::YFP background at day 8 of adulthood. Dots are means of individual experiments (≥10 worms per condition). The averages (+SEM, n ≥ 3) are shown as bar graphs. **p < 0.01, ***p < 0.001 (ANOVA).

(E) Motility of control and muscle-specific gfat-1 OE in unc-54P::Q35::YFP background at day 8 of adulthood when grown on control luciferase or gfat-1 RNAi. Means of individual experiments are displayed as dots (≥10 worms per condition). The averages (+SEM, n = 3) are shown as bar graphs. ***p < 0.001 (ANOVA).

(F) Motility of control and muscle-specific gfat-1 OE in unc-54P::Q35::YFP background at day 8 of adulthood when grown on control luciferase or atf-5 RNAi. A representative experiment from two repeats is displayed (n = 30 worms), ***p < 0.001 (ANOVA).

See also Figure S4.