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Comparison of specific activities of wild-type Lxmdh with S101, T141, and A164 mutant variants. The specific activities toward C1–C4 alcohols (A–D) were measured in 50 mM CHES buffer (pH 9.5) containing 3 mM NAD+ and 5 mM Mg2+ with the enzyme at 55 °C for 5 min. (E) S101, T141, and A164 are represented as a stick in the homology model.
