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. 2020 Feb 14;11:196. doi: 10.3389/fimmu.2020.00196

Figure 3.

Figure 3

Malnourished pigs had significantly reduced HRV-specific IgG and IgA antibody secreting cells (ASCs) post-AttHRV vaccination and numerically reduced ASC (HIFM pigs) post-VirHRV challenge in blood, duodenum, and ileum. HRV specific IgG (A) and IgA (B) ASCs in blood. HRV specific IgM (C), IgG (D), and IgA (E) ASCs in duodenum. Note 10× difference in y axis scale for C (IgM ASCs). Note 5× difference in y axis scale for E (IgA ASC). HRV specific IgM (F), IgG (G), and IgA (H) ASCs in ileum. Note 5× difference in y axis scale for H (IgA ASCs). Significant differences (*p < 0.05, ***p < 0.001) are indicated. Gnotobiotic pigs were transplanted with human infant fecal microbiota (HIFM) at 4 days of age. Pigs were fed deficient diet (Deficient) and sufficient diet (Sufficient). All the pigs were orally vaccinated twice with AttHRV on post transplantation day (PTD) 7 and 17, challenged with virulent human rotavirus (VirHRV) on PTD24/PVD17(7)/PCD0 and euthanized on PTD31/PVD24(14)/PCD7. PVD, post 1st (2nd) vaccination day; PCD, post-challenge day.