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. 2020 Jan 31;34:124–135. doi: 10.1016/j.molmet.2020.01.016

Figure 6.

Figure 6

Detection of irisin in rat and human serum by mass spectrometry (PRM mode). A: Coomassie-stained gels with boxed regions of interest removed for targeted mass spectrometry in the PRM mode; 300 μg total protein per sample was subjected to electrophoresis (5 × 60 μg). Only ∼270 μg total protein was available for sample HS 7b. B: PRM elution profile for the heavy AQUA irisin peptide (FIQEDVTTTR) in the rat (upper panel) and human (lower panel) sera. C: Corresponding profiles of the tryptic irisin peptide (FIQEDVTTTR). *Human samples: HS 6b (12 w training) and HS 7b (12 w training + acute exercise; details in Supplementary Table S1).