Table 1.

Antibodies used to detect irisin in this study and other research.

#	Manufacturer/vendor	Cat. #	Lot #	Immunogen	Laba/[Ref.]		Observed bands in plasma/serum	Remarks
Antibodies used in this study with previous results
A	Phoenix	G-067-29	01701–1	Recombinant irisin	FBN		See results
B	Erickson Labb	n/a	n/a	Recombinant irisin	FBN		See results
C	BioVision	6635	2A126635	Recombinant irisin	FBN		See results
D	AdipoGen	AG-25B-0027	A25481312/A27881602	Recombinant irisin	FBN Bos [7]		See results 25/12 kDa (native/+nebc), human	50 μg protein
					Bos [14]		22 kDa (+NEB), mouse	80–100 μg protein
E	Abgent	AP8746b	SA121121BK	FNDC5 C-terminus	FBN		n/a; see results	Used in tissue only
Antibodies used for irisin detection in other studies
	Abcam	Ab93373	Not specified	FNDC5 C-terminus	Bos [1]	32/20 kDa (native/+PNGd), human, mouse		Irisin peptide is not covered by this immunogen
					Bet [12]	32/24 kDa (native/+PNG), human
					Tai [39]	22 kDa (+PNG), human
	Phoenix	Not specified	Not specified	Recombinant irisin	Cho [13]	12 kDa (native), mouse		20 μL serum
	Phoenix	EK-067-52	Not specified	Recombinant irisin	Lei [37]	17 kDa (+NEB), human		Ab from ELISA kit
	BioTrend	A000170-01	01478/01483	Recombinant irisin	FBN [26]	15/12 kDa (native/+PNG), mouse		Ab discontinued
	Cell Sig Techn	Proprietary	N/A	unknown	Bos [10]	20 kDa (native), mouse

^aFBN: Leibniz Institute for Farm Animal Biology (FBN) Dummerstorf, Germany; Bos: Harvard University Medical School, Boston, MA, USA; Bet: National Institutes of Health, Bethesda, MD, USA; Tai: Chang Gung University College of Medicine, Taoyuan, Taiwan; Cho: Third Military Medical University, Chongqing, P.R. China; Lei: Center for Pediatric Research, University of Leipzig, Germany.

^bProduced in the laboratory of Dr. H. P. Erickson (Duke University, Durham, NC, USA).

^cNative = untreated serum; +NEB = samples deglycosylated with protein deglycosylation mix (NEB).

^d+PNG = samples deglycosylated with PNGase F.