Table 2.
Characteristics of the serum samples before and after processing.
| Serum samplesa | Original sample |
Sample after processingb |
Batch | Subsequent analysisc | |||
|---|---|---|---|---|---|---|---|
| Protein concentration (mg/mL) | Protein amount (mg/115 μL) | Retained volume (μL) | Retained protein |
||||
| mg | (%) | ||||||
| Human HS 2b | 54.41 | 6.26 | 70 | 1.02 | 16.2 | 2 | |
| Human HS 4a | 58.80 | 6.76 | 80 | 1.12 | 16,6 | 3 | |
| Human HS 4b | 53.99 | 6.21 | 90 | 1.21 | 19.5 | 3 | |
| Human HS 5a | 65.51 | 7.53 | 75 | 1.74 | 23.1 | 3 | |
| Human HS 5b | 62.54 | 7.19 | 100 | 1.20 | 16.6 | 3 | |
| Human HS 6b | 51.94 | 5.97 | 75 | 1.73 | 29.0 | 1 | |
| Human HS 6b (technical replicate) | 105 | 1.23 | 20.6 | 1 | MS (PRM) | ||
| Human HS 7b | 56.19 | 6.46 | 95 | 1.23 | 19.1 | 1 | MS (PRM) |
| Human HS 7b (technical replicate) | 75 | 1.79 | 27.7 | 1 | MS (PRM) | ||
| Human HS 7c | 54.92 | 6.32 | 70 | 1.02 | 16.1 | 2 | MS (DDA) |
| Rat | 45.89 | 5.28 | 50 | 2.04 | 38.7 | 3 | MS (DDA) |
| Rat (technical replicate) | 70 | 2.28 | 43.2 | 1 | MS (PRM) | ||
| Horse | 54.71 | 6.29 | 100 | 1.34 | 21.3 | 3 | |
a
See Supplemental Table S1 for detailed sample data.
b
Removal of albumin and IgG with ProteoExtract (Calbiochem); concentration of proteins with Amicon Ultra 0.5 Centrifugal Filter Devices (Merck).
c
MS: mass spectrometry; PRM: parallel reaction monitoring; DDA: data dependent acquisition.