Table 3.
Quantification of irisin in human and rat serum.
| Samplea | Remarks | Amount of peptide (48FIQEVDTTTR57; fmol) | Calculated irisin concentrationb (ng/mL serum) |
|---|---|---|---|
| HS 6b | N/D | – | |
| HS 7b | N/D | – | |
| HS 7b | Technical replicate | 0.42 | 0.091 |
| Repeated measurement | 0.70 | 0.153 | |
| Rat | 2.84 | 0.618 | |
| Repeated measurement | 3.60 | 0.783 | |
| Rat | Technical replicate | 4.20 | 0.914 |
| Repeated measurement | 4.35 | 0.946 |
N/D: Not detected.
a
See Supplemental Table S1 for detailed sample data.
b
fmoles of peptide x 25 kDa (MW of glycosylated irisin) x 8.696 (for 115 μL processed serum).