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. 2020 Jan 21;16:197–206. doi: 10.1016/j.omto.2020.01.003

Figure 5.

Figure 5

Effect of siRNA Gene Silencing on ERK1/2 Pathway through Western Blotting Analysis on FTC-133 and 8305C Cell Line Cultures

(A) Representative immunoblots of ERK1/2 expression levels in total cellular lysates from scrambled FTC-133 and 8305C human thyroid cancer cell lines and 10 nM siRNA-TfR1-transfected cells for 48 h. (B) Densitometric analysis of ERK1 and ERK2 expression levels in response to siRNA-TfR1 gene silencing in transfected FTC-133 and 8305 human thyroid cancer cell lines, when compared with scrambled ones, used as control (performed after normalization with β-tubulin). (C) Representative immunoblot of c-Myc expression levels in total cellular lysates from scrambled FTC-133 and 8305C human thyroid cancer cell lines and 10 nM siRNA-TfR1 transfected cells for 48 h. (D) Densitometric analysis of c-Myc (performed after normalization with β-tubulin) in response to siRNA-TfR1 transfection, when compared with scrambled ones, used as control. Results are expressed as the mean ± SD of the values of five separate experiments performed in triplicate. *p < 0.05 for differences versus controls.