Table 1.

Effect of RNA Interference on the Percentage of Cell Viability in FTC-133 Cells

Treatment	% of Inhibition of Cell Viability
	24 h	48 h	72 h
2 nM scrambled	99.01 ± 1	98.77 ± 1	99.31 ± 1
4 nM scrambled	98.97 ± 2	97.89 ± 2	98.72 ± 2
6 nM scrambled	98.45 ± 2	98.34 ± 3	97.44 ± 2
8 nM scrambled	97.86 ± 3	98.78 ± 2	99.33 ± 1
10 nM scrambled	97.93 ± 2	97.88 ± 2	97.01 ± 2
2 nM siRNA	94.99 ± 1	91.01 ± 1	89.58 ± 3
4 nM siRNA	86.78 ± 1*	83.06 ± 3*	81.99 ± 1*
6 nM siRNA	78.67 ± 3*	66.78 ± 2*	65.97 ± 2*
8 nM siRNA	70.24 ± 2*	51.55 ± 1*	51.34 ± 1*
10 nM siRNA	64.01 ± 2*	42.88 ± 3*	41.99 ± 2*

The effect of RNA interference on the percentage of cell viability was determined though an MTT test in human follicular human thyroid cancer cell line cultures (FTC-133) transfected with different concentrations (2, 4, 6, 8, and 10 nM) of scrambled control or siRNA-TfR1 for 24, 48, and 72 h. Results are expressed as the mean ± SD of the values of five separate experiments performed in triplicate. *p < 0.05 for differences versus controls.