Table 2.

Effect of RNA Interference on the Percentage of Cell Viability for 8305C Cells

Treatment	% of Inhibition of Cell Viability
	24 h	48 h	72 h
2 nM scrambled	98.91 ± 1	99.01 ± 1	97.96 ± 2
4 nM scrambled	99.06 ± 1	98.67 ± 2	98.01 ± 2
6 nM scrambled	97.78 ± 2	99.24 ± 1	98.92 ± 1
8 nM scrambled	97.91 ± 3	98.46 ± 2	97.05 ± 3
10 nM scrambled	98.72 ± 2	97.88 ± 2	98.81 ± 2
2 nM siRNA	95.23 ± 2	93.87 ± 1	83.89 ± 3
4 nM siRNA	88.34 ± 2	85.89 ± 1*	82.11 ± 1*
6 nM siRNA	80.66 ± 1*	78.34 ± 1*	76.08 ± 1*
8 nM siRNA	76.22 ± 2*	71.88 ± 3*	68.83 ± 3*
10 nM siRNA	73.34 ± 3*	49.31 ± 1*	48.77 ± 2*

The effect of RNA interference on the percentage of cell viability was determined though an MTT test in human anaplastic human thyroid cancer cell line cultures (8305C) transfected with different concentrations (2, 4, 6, 8, and 10 nM) of scrambled control or siRNA-TfR1 for 24, 48, and 72 h. Results are expressed as the mean ± SD of the values of five separated experiments performed in triplicate. *p < 0.05 for differences versus controls.