Table 2.
Treatment | % of Inhibition of Cell Viability |
||
---|---|---|---|
24 h | 48 h | 72 h | |
2 nM scrambled | 98.91 ± 1 | 99.01 ± 1 | 97.96 ± 2 |
4 nM scrambled | 99.06 ± 1 | 98.67 ± 2 | 98.01 ± 2 |
6 nM scrambled | 97.78 ± 2 | 99.24 ± 1 | 98.92 ± 1 |
8 nM scrambled | 97.91 ± 3 | 98.46 ± 2 | 97.05 ± 3 |
10 nM scrambled | 98.72 ± 2 | 97.88 ± 2 | 98.81 ± 2 |
2 nM siRNA | 95.23 ± 2 | 93.87 ± 1 | 83.89 ± 3 |
4 nM siRNA | 88.34 ± 2 | 85.89 ± 1* | 82.11 ± 1* |
6 nM siRNA | 80.66 ± 1* | 78.34 ± 1* | 76.08 ± 1* |
8 nM siRNA | 76.22 ± 2* | 71.88 ± 3* | 68.83 ± 3* |
10 nM siRNA | 73.34 ± 3* | 49.31 ± 1* | 48.77 ± 2* |
The effect of RNA interference on the percentage of cell viability was determined though an MTT test in human anaplastic human thyroid cancer cell line cultures (8305C) transfected with different concentrations (2, 4, 6, 8, and 10 nM) of scrambled control or siRNA-TfR1 for 24, 48, and 72 h. Results are expressed as the mean ± SD of the values of five separated experiments performed in triplicate. *p < 0.05 for differences versus controls.