Figure 3.

KDM3A Is Highly Expressed in Chronic Renal Fibrosis and miR-101a Targets and Negatively Regulates the Expression of KDM3A

(A) Targeting binding sequence between miR-101a and KDM3A analyzed using an online website. CCNE1, G₁/S-specific cyclin E1. (B) The binding of miR-101a to KDM3A confirmed by a dual-luciferase reporter assay. (C) Western blot analysis of KDM3A protein in UUO mice injected with miR-101a agomir. (D) KDM3A expression in UUO mice injected with miR-101a agomir determined by qRT-PCR. (E) KDM3A expression in AA-incubated cells transfected with miR-101a mimic detected by qRT-PCR. In (B) and (C), *p < 0.05 versus sham-operated mice; ^#p < 0.05 versus mice treated with UUO + agomir-NC. In (D) and (E), *p < 0.05 versus control cells; ^#p < 0.05 versus AA-incubated cells transfected with mimic-NC. The above data were all measurement data and are expressed as mean ± standard deviation. Data between two groups were compared by an unpaired t test. The cell experiment was conducted in triplicate.