Figure 8.

Upregulated YAP/TGF-β2 or Inhibited TGIF1 Facilitates the Inhibitory Effect of miR-101a on Chronic Renal Fibrosis

(A) Western blot analysis of YAP1, TGF-β2, and TGIF1 proteins. *p < 0.05 versus control cells; ^#p < 0.05 versus AA-incubated cells transfected with mimic-NC. (B) Western blot analysis of Col1a1, fibronectin, and α-SMA proteins in cells. *p < 0.05 versus AA-incubated cells transfected with miR-101a mimic and oe-NC; ^#p < 0.05 versus AA-incubated cells transfected with miR-101a mimic and sh-NC. (C) Immunohistochemistry analysis of Col1a1, fibronectin, and α-SMA proteins in kidney tissues of mice (original magnification, ×200). (D) Masson’s trichrome staining of kidney tissues of mice (original magnification, ×200). The above data were all measurement data and are expressed as mean ± standard deviation. Data among multiple groups were analyzed by one-way ANOVA, followed by a Tukey’s multiple comparisons post-test. The cell experiment was conducted in triplicate.