Table 2.
Primer Sequences for qRT-PCR
| Gene | Sequences (5′→3′) |
|---|---|
| m-KDM3A | F: CGTTACTAAGAAAGACTTGAAGGTG |
| R: CCAGGTTATGCTCTACTAAAAATGC | |
| h-KDM3A | F: ATGCTGCAAAGGACACGG |
| R: GAACTCCATACTCTTGATGAAGACG | |
| m-miR-101a | F: GAGGGGTACAGTACTGATA |
| R: TGCGTGTCGTGGAGTC | |
| h-miR-101a | F: TCCCCCGGGCCAGAGGTT GTAACGTTGTCTAT |
| R: GAAACCCAGCAGACAAAGCT TTGTTGCCTAACGAAC | |
| m-U6 | F: GCATGACGTCTGCTTTGGA |
| R: CCACAATCATTCTGCCATCA | |
| h-U6 | F: CTCGCTTCGGCAGCACA |
| R: AACGCTTCAGGAATTTGCGT | |
| m-YAP1 | F: CTGCGTGCAGAAATGCTACTG |
| R: AGCCGTAGAGTAATGGTGGATAG | |
| h-YAP1 | F: CCTGATGGATGGGAACAAGC |
| R: GCACTCTGACTGATTCTCTGG | |
| m-TGF-β2 | F: GGAGGTGATTTCCATCTACAA |
| R: GGGAGATGGTAAGTCTTTGGGA | |
| h-TGF-β2 | F: CCCACATCTCCTGCTAATGT |
| R: GCTGAGTGTCTGAACTCCAT | |
| m-TGIF1 | F: AGAGGCAATCTGCCCAAGG |
| R: GGGATAGGCGTTGTATCTGTG | |
| h-TGIF1 | F: GCTGTCCCAGCAAACACACC |
| R: TTCTCAGCATGTCAGGGAGGA | |
| m-β-actin | F: ACGTGCCGCCTGGAGAAAC |
| R: GTCCTCAGTGTAGCCCAAGATGC | |
| h-β-actin | F: GTGAAGGTGACAGCAGTCGGTT |
| R: GAAGTGGGGTGGCTTTTAGGA |
qRT-PCR, quantitative reverse transcription polymerase chain reaction; m, mouse; h, human; KDM3A, lysine (K)-specific demethylase 3A; YAP1, Yes-associated protein 1; TGF-β2, transforming growth factor β2; TGIF1, TGFB-induced factor homeobox 1; F, forward; R, reverse.