Figure 3.

Glucosinolates (GLSs) accumulated in Nicotiana benthamiana upon transient expression of CYP79C1 and CYP79C2 in combination with the aromatic core pathway. (A) Scheme of introduced enzymes including different CYP79 enzymes (pink) and remaining “aromatic” core pathway enzymes as well as the stimulating enzyme APK2 (green). The combination without CYP79 represents the negative control and the combination with CYP79A2 is the positive control. (B) GLSs accumulated with constructs including CYP79C2. (C) GLSs accumulated with constructs including CYP79C1. (D) GLSs accumulated with constructs including CYP79A2. (E) GLSs accumulated with the negative control constructs without CYP79 co-expressed. Data of each box plot represent nine biological replicates in nanomole per gram fresh weight. n.d. represents not detected. Data in (B–E) are box-and-whisker representations indicating the 10th (lower whisker), 25th (base of box), 75th (top of box), and 90th (top whisker) percentiles. The line within the box is the median and outliers are not shown. Exact values are listed in Supplementary Table S5. P-values (two-sided Student's t-test) used to compare the same type of GLS yields among B, C, and E are listed in Supplementary Table S6.