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. 2020 Feb 14;11:57. doi: 10.3389/fpls.2020.00057

Table 1.

The substrate specificity and affinity of the CYP79 family members.

CYP79 enzyme Substrate specificity Substrate affinity (KM) Plant species Locus Reference
CYP79A1 Tyrosine KM, = 220 µM Sorghum bicolor LOC8061413 Halkier et al., 1995
CYP79A2 Phenylalanine KM, = 6.7 µM Arabidopsis thaliana AT5G05260 Wittstock and Halkier, 2000
CYP79A8 Leucine Not tested Hordeum vulgare ACJ70085 Knoch et al., 2016
CYP79A12 Leucine Not tested Hordeum vulgare ACM24114 Knoch et al., 2016
CYP79A61 Phenylalanine,
Tryptophan		 KM, Phe = 117.2 μM,
KM, Trp = 150.2 µM		 Zea mays AKJ87843 Irmisch et al., 2015
CYP79A118
(N-terminal truncated)		 Tyrosine,
Phenylalanine,
Tryptophan		 KM, Tyr = 456 µM,
KM, Phe = 21690 μM,
KM, Trp = 24150 μM		 Taxus baccata ART92261 Luck et al., 2017
CYP79B1 Tryptophan KM, = 29 µM Sinapis alba AAD03415 Naur et al., 2003
CYP79B2 Tryptophan KM, = 21 µM Arabidopsis thaliana AT4G39950 Mikkelsen et al., 2000
CYP79B3 Tryptophan Not tested Arabidopsis thaliana AT2G22330 Zhao et al., 2002
CYP79C1 Valine,
Phenylalanine,
Leucine,
Isoleucine		 Not tested
Not tested
Not tested
Not tested		 Arabidopsis thaliana AT1G79370 This study
CYP79C2 Phenylalanine,
Leucine,
Isoleucine,
Tryptophan,
Tyrosine		 Not tested
Not tested
Not tested
Not tested
Not tested		 Arabidopsis thaliana AT1G58260 This study
CYP79D1 Valine,
Isoleucine		 KM, Val = 2200 µM,
KM, Ile = 1300 µM		 Manihot esculenta AAV97889 Andersen et al., 2000
CYP79D2 Valine,
Isoleucine		 Not tested
Not tested		 Manihot esculenta AAV97888 Mikkelsen and Halkier, 2003
CYP79D3 Valine,
Isoleucine		 Not tested
Not tested		 Lotus japonicus AAT11920 Forslund et al., 2004
CYP79D4 Valine,
Isoleucine		 Not tested
Not tested		 Lotus japonicus AAT11921 Forslund et al., 2004
CYP79D6v3 Phenylalanine,
Leucine,
Isoleucine,
Tryptophan,
Tyrosine		 KM, Phe = 744 μM,
KM, Leu = 447 μM,
KM, Ile = 526 µM,
KM, Trp = 1427 μM,
KM, Tyr = 1828 µM		 Populus trichocarpa AHF20912 Irmisch et al., 2013a
CYP79D6v4 Phenylalanine,
Leucine,
Isoleucine,
Tryptophan,
Tyrosine		 Not tested
Not tested
Not tested
Not tested
Not tested		 Populus nigra AHI88992 Irmisch et al., 2013b
CYP79D7v2 Phenylalanine,
Leucine,
Isoleucine,
Tryptophan		 KM, Phe = 2901 μM,
KM, Leu = 633 μM,
KM, Ile = 851 µM,
KM, Trp = 285 μM		 Populus trichocarpa AHF20913 Irmisch et al., 2013a
CYP79D60 Phenylalanine,
Isoleucine,
Leucine,
Tryptophan,
Tyrosine		 KM, Phe = 580 μM,
KM, Ile = 1280 μM,
KM, Leu = 230 μM,
KM, Trp = 2740 μM,
KM, Tyr = 6090 μM,		 Erythroxylum fischeri AOW44273 Luck et al., 2016
CYP79D61 Phenylalanine,
Isoleucine,
Leucine,
Tryptophan,
Tyrosine		 Not tested
Not tested
Not tested
Not tested
Not tested		 Erythroxylum fischeri AOW44271 Luck et al., 2016
CYP79D62 Phenylalanine,
Isoleucine,
Leucine,
Tryptophan,
Tyrosine		 KM, Phe = 670 μM,
KM, Ile = 3270 μM,
KM, Leu = 590 μM,
KM, Trp = 1090 μM,
KM, Tyr = 4990 μM,		 Erythroxylum coca AOW44274 Luck et al., 2016
CYP79D63 Tryptophan KM, = 480 µM Erythroxylum coca AOW4427 Luck et al., 2016
CYP79E1 Tyrosine Not tested Triglochin maritima AF140609_1 Nielsen and Møller, 2000
CYP79E2 Tyrosine Not tested Triglochin maritima AF140610_1 Nielsen and Møller, 2000
CYP79F1 1homoMet,
2homoMet,
3homoMet,
4homoMet,
5homoMet,
6homoMet,		 Not tested
KM, 2homoMet = 34 µM,
KM, 3homoMet = 37 µM,
KM, 4homoMet = 194 µM,
KM, 5homoMet = 216 µM,
KM, 6homoMet = 74 µM		 Arabidopsis thaliana AT1G16410 Chen et al., 2003
CYP79F2 5homoMet,
6homoMet,		 KM, 5homoMet = 374 µM,
KM, 6homoMet = 26 µM		 Arabidopsis thaliana AT1G16400 Chen et al., 2003

1homoMet, homomethionine; 2homoMet, dihomomethionine; 3homoMet, trihomomethionine; 4homoMet, tetrahomomethionine; 5homoMet, pentahomomethionine; 6homoMet, hexahomomethionine.