Figure 1.

Experimental approach of CD1b tetramer study. (A) Flow cytometric study of 150 Peruvian subjects was conducted with two arms, existing of expanded T cells and direct ex vivo peripheral blood mononuclear cells (PBMC). (B) Chemical structures of natural methoxy mycolic acid from Mtb and glucose monomycolate from Rhodococcus equi, which were both used to load CD1b tetramers. (C) Validation of CD1b tetramers using PBMC from a Boston blood bank donor, spiked with the relevant antigen specific T cell line. (D) T cell expansion did not dramatically alter the TCR variable (V) gene usage when compared to PBMC from the same blood donor.