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. 2020 Feb 14;11:199. doi: 10.3389/fimmu.2020.00199

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Copyright © 2020 Lopez, Iwany, Suliman, Reijneveld, Ocampo, Jimenez, Calderon, Lecca, Murray, Moody and Van Rhijn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantification of CD1b-mycolic acid and CD1b-glucose monomycolate tetramer⁺ T cells in a Peruvian TB cohort. (A) Expanded peripheral blood mononuclear cells were gated based on forward and side scatter, as well as CD3 expression and low autofluorescence, which was determined in the FITC channel. (B) Representative flow cytometry plots of four of the 150 members of the cohort after staining with CD1b-mock, CD1b-MA, and CD1b-GMM tetramers after pre-gating as shown in (A). Numbers indicate percent cells in gate. (C) Frequencies of tetramer⁺ T cells among all subjects of the Peruvian cohort, analyzed by TB disease status. Medians and interquartile ranges of tetramer⁺ T cells are depicted as a percent of total CD3⁺ cells.