Figure 3.

GEM T cells and LDN5-like T cells are simultaneously present in the T cell repertoire in Peruvian subjects. (A) After pre-gating as in Figure 2A, detection of GEM T cells and LDN5-like T cells was based on CD1b-GMM tetramer and antibodies against CD4 and TCR variable segments TRAV1-2 or TRBV4-1. (B) Quantification of the proportion of frequencies of GEM T cells, LDN5-like T cells and other CD1b-GMM tetramer⁺ T cells that lack defining variable region segments in active TB patients (n = 34), IGRA⁺ (latent infection) (n = 38) household contacts and IGRA⁻ (uninfected) (n = 43) household contacts. (C) Median proportions of T cell subsets of CD1b-GMM tetramer⁺ T cells analyzed according to TB disease status are shown.