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qPCR of 2D- and 3D-cultured hiPSC-CMs and CF. Shown are fold-changes of expression normalized to GAPDH expression. (A) Comparison of gene expression of pure hiPSC-CMs (CM) in 3D vs. 2D culture. (B) Comparison of co-cultured hiPSC-CMs and CF in 3D vs. pure hiPSC-CMs in 3D. (C) Comparison of pure CF in 3D vs. CF monolayer culture in 2D. N = 3, *p < 0.05, **p < 0.01, ***p < 0.001.
