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. 2020 Feb 14;11:196. doi: 10.3389/fmicb.2020.00196

TABLE 7.

Changes in RNA expression levels of biofilm formation-related genes in 11 clinical isolates with radezolid or linezolid treatment.

Biofilm formation-related genes
agg ahrC asa1 atn cylA eep ebpA epaI epaOX esp fsrA gelE hyl relA relQ srtA
16C1
RZD 0.578 0.108a 0.784 1.358 0.388c – 0.864 0.897 1.062 0.113d 1.234 0.687 1.657 0.218f 0.359 0.687
LZD 0.864 0.368 0.895 1.295 0.664 – 1.234 1.065 1.364 0.268 1.498 0.892 1.552 0.458 0.487 0.734
16C35
RZD 0.854 0.169b 1.285 1.069 0.295 0.965 0.937 1.298 1.118 0.165d 0.931 0.885 – 0.311f 0.365 1.058
LZD 1.068 0.293 1.154 1.035 0.431 1.262 1.364 1.105 0.997 0.378 0.854 1.158 – 0.524 0.487 1.364
16C51
RZD 0.814 0.167 1.025 1.168 0.505 – 1.035 0.954 1.548 0.075d 0.875 – – 0.309f 0.224 0.564
LZD 0.962 0.208 1.132 0.864 0.598 – 1.125 1.264 1.068 0.119 0.965 – – 0.524 0.368 0.635
16C102
RZD – 0.263b – 0.864 – 0.597 0.824 0.954 0.931 0.138d 0.768 0.687 – 0.269f 0.218h 0.694
LZD – 0.597 – 1.126 – 0.854 0.789 1.158 0.865 0.367 0.895 0.885 – 0.486 0.531 0.954
16C124
RZD – 0.597 1.164 1.106 0.167c 0.364 0.764 0.854 0.954 0.107e 0.964 0.989 – 0.208 0.497 1.065
LZD – 0.631 0.965 1.357 0.284 0.543 0.805 0.762 1.035 0.485 1.165 1.164 – 0.294 0.551 1.321
16C138
RZD – 0.156 0.881 1.265 0.464 – 1.068 0.924 1.268 0.255d 1.123 – – 0.368f 0.305h 1.246
LZD – 0.198 1.098 1.067 0.657 – 1.267 1.157 0.954 0.495 1.065 – – 0.542 0.687 1.098
16C152
RZD 0.954 0.468 1.065 1.196 0.365c 1.354 0.854 1.094 1.354 0.073e 0.882 0.724 – 0.097g 0.267h 0.769
LZD 1.367 0.598 1.298 0.932 0.697 1.158 1.063 1.265 1.165 0.396 0.854 0.658 – 0.264 0.576 0.854
16C166
RZD – 0.097b 0.854 1.267 – 0.862 0.962 1.164 1.264 – 0.931 – – 0.168f 0.186h 0.367
LZD – 0.167 0.801 1.065 – 1.065 1.264 0.938 1.129 – 1.095 – – 0.291 0.367 0.431
16C201
RZD – 0.157b 0.652 0.597 – – 0.531 0.789 0.631 – 0.934 – – 0.103g 0.158 0.687
LZD – 0.269 0.896 0.786 – – 0.764 0.894 0.835 – 1.264 – – 0.324 0.263 1.357
16C289
RZD 0.764 0.298 1.068 1.267 0.368c 0.714 0.965 1.158 0.958 0.085e 0.867 0.534 2.674 0.208f 0.296h 0.974
LZD 0.878 0.367 1.165 1.152 0.587 1.257 1.247 1.036 1.168 0.298 0.906 0.738 1.687 0.593 0.687 1.265
16C353
RZD – 0.394 1.267 0.954 0.464 – 0.854 0.964 1.068 0.069e 0.768 – – 0.082g 0.158i 0.875
LZD – 0.485 1.597 1.264 0.568 – 0.834 1.167 0.915 0.468 0.934 – – 0.368 0.497 1.068

RZD, radezolid; LZD, linezolid. Radezolid and linezolid were used at 1/4× their minimum inhibitory concentrations (MICs). The RNA levels were detected by RT-qPCR, with untreated isolate as the reference strain (mRNA level = 1.0). −, not detected. aahrC: RZD vs. LZD, P < 0.01; bahrC: RZD vs. LZD, P < 0.05; ccylA: RZD vs. LZD, P < 0.05; desp: RZD vs. LZD, P < 0.05; eesp: RZD vs. LZD, P < 0.01; frelA: RZD vs. LZD, P < 0.05; grelA: RZD vs. LZD, P < 0.01; hrelQ: RZD vs. LZD, P < 0.05; irelQ: RZD vs. LZD, P < 0.01.