Figure 6.

Reduction of Individual Protein Translation Rates Is Defined by the Extent of Global Translation Attenuation

(A) Median relative translation for cells treated with DMSO, 0.25 μM, 1 μM, or 6 μM thapsigargin (Tg) for 2.5 h (left panel) or DMSO, 0.75 μM, or 2 μM Torin1 for 9 h (right panel).

(B) Heatmap showing Z scores of relative translation rates for individual proteins across treatments (Z scores were calculated for each experiment). Clustering of samples were performed with Euclidean distance. Relative median translation rates compared to control are plotted on top of the heatmap for each sample.

(C) Standardized (Z score) relative translation rates for the subset of proteins showing a decrease in translation correlating with global translation attenuation after titration of treatments. Clustering was performed on data from (B) and values of the most prominent cluster plotted for each treatment. Black lines indicate averaged curves from all displayed proteins.

(D) Median relative translation rates of cells treated with indicated concentrations of 4EGI.

(E) Heatmap displaying correlation of samples treated with different concentrations of either 4EGI, thapsigargin (Tg) or Torin1. Values represent Euclidean distance between samples. Clustering was performed over Euclidean distance. Apparent clusters are marked in red.

(F) Heatmap displaying standardized relative translation values (Z score) for individual proteins following 4EGI treatment.

(G) Standardized translation rates (Z score) for all proteins showing linear behavior of translation repression upon 4EGI titration (Figure S6B). See also Figure S6 and Table S5.