REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
ACTB	SantaCruz	Cat#sc-69879; RRID:AB_1119529
EIF4EBP1	Cell Signaling Technologies	Cat#9644; RRID:AB_2097841
EIF2S1	Abcam	Cat#ab5369; RRID:AB_304838
p-EIF2S1(S51)	Abcam	Cat#ab32157; RRID:AB_732117
IRDye 680RD anti-mouse	Li-Cor	Cat# 925-68070; RRID:AB_2651128
IRDye 800CW anti-rabbit	Li-Cor	Cat# 925-32211; RRID:AB_2651127
Chemicals, Peptides, and Recombinant Proteins
Thapsigargin	Abcam	Cat#ab120286
2-Chloracetamide	Sigma Aldrich	Cat#C0267
Arginine 10	Cambridge Isotope Laboratories	Cat#CNLM-539-H-PK
Lysine 8	Cambridge Isotope Laboratories	Cat#CNLM-291-H-PK
Sodium (meta)arsenite	Sigma Aldrich	Cat#S7400
ISRIB	Sigma Aldrich	Cat#SML0843
4EGI-1	Selleckchem	Cat#S7369
TMT reagents	Thermo Fisher Scientific	Cat#90111, Cat#A37724, Cat#90061
Torin1	Cell Signaling Technologies	Cat#14379
Critical Commercial Assays
μBCA microplate assay	Thermo Fisher Scientific	Cat#23235
Deposited Data
RAW and quantified data	This paper/PRIDE	PXD014377 and PXD015438
Ribosome profiling and RNaseq data	Thoreen et al., 2012, Reid et al., 2014, Paolini et al., 2018, Andreev et al., 2015	https://doi.org/10.1038/nature11083, https://doi.org/10.1016/j.cell.2014.08.012, https://doi.org/10.1371/journal.pone.0193790, https://doi.org/10.7554/eLife.0397
SwissProt Human protein database	SwissProt	https://www.uniprot.org/downloads
Experimental Models: Cell Lines
Human: HeLa		N/A
Software and Algorithms
Python 3.6	Pyhton Consortium	https://www.python.org/; RRID:SCR_008394
pandas 0.23.4	McKinney, 2010	https://scipy.org/
Proteome Discoverer 2.2	ThermoFisher Scientific	Cat#OPTON-30795; RRID:SCR_014477
MaxQuant 1.6	Cox and Mann, 2008	https://www.maxquant.org/; RRID:SCR_014485
Perseus 1.6.2.3	Tyanova et al., 2016	https://www.maxquant.org/; RRID:SCR_015753
Numpy 1.15.4	van der Walt et al., 2011	https://scipy.org/; RRID:SCR_008633
Cytoscape 3.5.1	Shannon et al., 2003	https://cytoscape.org/; RRID:SCR_003032
BiNGO 3.0.3	Maere et al., 2005	https://www.psb.ugent.be/cbd/papers/BiNGO/Home.html; RRID:SCR_005736
EnrichmentMap 3.1.0	Merico et al., 2010	http://apps.cytoscape.org/apps/enrichmentmap; RRID:SCR_016052
Origin Pro 2018	OriginLab	https://www.originlab.com/2018; RRID:SCR_014212
ReactomeFI 6.1.0	Wu and Haw, 2017	http://apps.cytoscape.org/apps/reactomefiplugin
matplotlib 3.0.1	Hunter, 2007	https://scipy.org/; RRID:SCR_008624
scikit-learn 0.20.1	Pedregosa et al., 2011	https://scikit-learn.org/stable/index.html; RRID:SCR_002577
Other
QExactive HF Orbitrap MS	Thermo Fisher Scientific	Cat#IQLAAEGAAPFALGMBFZ
Orbitrap Fusion Lumos Tribrid MS	Thermo Fisher Scientific	Cat#IQLAAEGAAPFADBMBHQ