Figure 2.

H2AK119ub1 Is Essential for PRCs-Mediated Transcriptional Repression

(A) Volcano plots of –log10 (p value) against log2 fold change representing the differences in gene expression, related to RNA-seq analysis, in the indicated cell lines upon EtOH treatment (−OHT). Upregulated (red) and downregulated (green) genes are highlighted.

(B) As in (A) upon OHT treatment (+OHT).

(C) Venn diagrams showing the overlap of upregulated genes between the indicated cell lines.

(D) As in (C) for downregulated genes.

(E) Scatterplot showing the relationship between log2 fold changes (FC) between the indicated cell lines at RING1B target loci. R2 represents the coefficient of determination of linear regression. Genes with promoters (±2.5 kb around transcription start site [TSS]) containing H2AK119ub1 peaks are highlighted in red.

(F) Barplots showing the percentage of upregulated (left) or downregulated (right) genes with promoters (±2.5 kb around TSS) containing H2AK119ub1 peaks in the indicated cell lines.

(G) Volcano plots of –log10 (p value) against log2 fold change representing the differences in gene expression, related to RNA-seq analysis, in EED^fl/fl versus EED^−/− ESCs. Upregulated (red) and downregulated (green) genes are highlighted.

See also Figure S1B.