Figure 6.

H2AK119ub1 Affects RIN1GB Chromatin Stability

(A) Heatmaps representing normalized RING1B ChIP-seq intensities ±8 kb around the center of RING1B target loci in the indicated cell lines.

(B) Boxplots representing RING1B ChIP-seq CPMK levels in the indicated cell lines at RING1B target loci.

(C) ChIP-qPCR analysis of HA in the indicated cell lines at five specific polycomb targets and one intergenic region. Parental cells served as a negative control.

(D) Western blot analysis with the indicated antibodies of soluble and insoluble protein fractions obtained from the specified cell lines upon 72 h of treatment with OHT (+OHT) or EtOH (−OHT). GAPDH and histone H3 were used as positive controls for the soluble and insoluble fractions, respectively.

(E) Heatmaps representing normalized RING1B ChIP-seq intensities ±8 kb around the center of RING1B target loci in the indicated cell lines.

(F) Boxplots representing RING1B ChIP-seq CPMK levels in the indicated cell lines at RING1B target loci.