FIGURE 1.

Different processing methods of positive blood culture broth. Dilution method was done with dilution steps up to 10^{– 4} in CAMHB. Dilutions were directly used for DOT-MGA. Lysis/centrifugation method was done according to the protocol of MBT Sepsityper kit (Bruker Daltonik GmbH, Bremen, Germany; version prior to introduction of rapid workflow and improved formulation), but only 100 μl lysis buffer was used. Differential centrifugation was performed with a first centrifugation step of 2 min at 2000 rpm and a second centrifugation step of 2 min at 13,000 rpm. Obtained pellets were resuspended and a standardized 0.5 McFarland suspension was prepared. Two different dilutions were used for DOT-MGA.