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. 2020 Feb 14;11:95. doi: 10.3389/fmicb.2020.00095

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Copyright © 2020 Kaundal, Deep, Kaur and Thakur.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Oligomeric state and binding kinetics of YeeF-CT, YezG, and their interactions. Sedimentation coefficient distribution (s) of YeeF-CT, YezG, and YeeF- CT/YezG are shown in panels A–C, respectively. (A,B) YeeF-CT and YezG exist predominantly as a homodimer and monomer in solution, respectively. (C) YeeF-CT/YezG associate to form a heterotetramer in solution. Peak I and II correspond to excess YezG and YeeF-CT, respectively. Peak III corresponds to YeeF-CT/YezG complex. In all the protein samples, a minor concentration-dependent higher order oligomer population is also observed. (D) A representative isotherm profile of YeeF-CT and YezG interactions. The upper panel represents the calorimetric titration and the lower panel represents derived binding isotherm plotted versus the molar ratio of the YezG.