Arabidopsis Plants Expressing N-terminally Truncated AtTSPO Are More Affected by Water Loss than Plants Overexpressing Full-Length AtTSPO
(A) Percentage water loss after 120 min from soil-grown 17-day-old Arabidopsis (whole aerial parts) grown in a phytotron (average temperature 20°C, approx. 65% humidity, 16 h photoperiod, ∼120 μmol.m−2.s−1) and dehydrated under light in the same conditions. WT, wild-type; KO, transfer DNA insertional AtTSPO knockout line; p35S::AtTSPO, AtTSPO-overexpressing line; p35S::AtTSPOH91A, transgenic line stably overexpressing AtTSPO harboring point mutation H91A; p35S::Venus-AtTSPOΔN, line overexpressing Venus-tagged N-terminally truncated AtTSPO. Pooled measurements from five rosettes coming from at least two pots (distributed randomly in the phytotron) are shown, and red horizontal lines define the means for each dataset. Statistical significance was assessed by one-way ANOVA followed by Tukey's tests (*p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant).
(B) Western blot of total protein extracts from rosettes assayed in (A). Per genotype, probed were two replicates (independent pots).
(C) Stomatal conductance of intact leaves from soil-grown mature plants under phytotron conditions. Genotypes are as in (A). Four plants (2–3 rosette leaves per plant) were tested for each line. Individual measurements are shown and red horizontal lines define the means for each dataset. Statistical analysis was conducted as in (A), with **p < 0.01.
(D) Western blot of total protein extracts from leaves assayed in stomatal conductance experiments.