Figure 7.

A Small Subset of PRC1 Target Genes Are Not Fully Derepressed Following Loss of PRC1 Catalysis

(A) A violin plot comparing log2 fold changes in expression (cRNA-seq) of PRC1-repressed genes that are partially (n = 241) or fully derepressed (n = 2,241) in PRC1^CPM as compared to PRC1^CKO ESCs following OHT treatment in Pcgf4^−/−;Pcgf2^fl/fl, PRC1^CPM, and PRC1^CKO ESC lines.

(B) A boxplot of expression levels (cRNA-seq) of genes described in (A) in PRC1^CPM and PRC1^CKO ESCs. P values denote statistical significance calculated by a Wilcoxon rank sum test.

(C) A genomic snapshot of a PRC1-repressed gene that is fully derepressed in OHT-treated PRC1^CPM as compared to PRC1^CKO ESCs, showing gene expression (cRNA-seq) in PRC1^CKO and PRC1^CPM ESCs.

(D) As in (C) for a PRC1-repressed gene that is partially derepressed in OHT-treated PRC1^CPM as compared to PRC1^CKO ESCs.

(E) Genomic snapshots for genes described in (C) and (D) showing cChIP-seq for PRC1 (RING1B, H2AK119ub1, and PCGF2) and PRC2 (SUZ12 and H3K27me3) in PRC1^CPM ESCs.

See also Figure S7.