FIGURE 5.

Puerarin protected against HG-induced injury in podocytes via HMOX-1/Sirt1. Cells were pre-incubated with puerarin for 15 min, and then incubated with HG (30 mM) for 48 h. (A) The mRNA expression of HMOX-1, and Sirt1 in podocytes without HG or Puerarin was determined by RT-PCR. (B) The expression of HMOX-1 and Sirt1 in podocytes without HG or Puerarin was determined by western blotting. (C,D) Analysis of apoptosis rates by flow cytometry and quantification. Data are expressed as the mean ± SEM (n = 3). (E) Whole cell extracts were immunoprecipitated with LKB1 antibody or an equal amount of rabbit IgG and blotted with acetylated-lysine (Acetyl) antibody and anti-LKB1 antibody. ***P < 0.001 vs. HG; ###P < 0.001 vs. HG + Pue 10.