Figure 2.

CISAL Promotes Mitochondrial Fission and Cisplatin Sensitivity in TSCC Cells through BRCA1

(A and B) CAL-27 and SCC-9 cells were treated with shRNA against CISAL. Mitochondrial fission and CYT c distribution was detected by staining with MitoTracker Red and antibodies against CYT c, respectively (A); cell apoptosis was detected using flow cytometry (B).

(C) CAL-27 and SCC-9 cells stably expressing two different shRNAs against CISAL (shCISAL2 and shCISAL5) were treated with cisplatin and their RNA was extracted and analyzed for mRNA profiling. Heatmap (upper panel) and Venn diagrams (lower panel) depict differentially expressed mRNAs in cisplatin-treated cells stably expressing shCISAL (fold change ≥2.0). Blue to red color gradation is based on the ranking of each condition from minimum (blue) to maximum (red).

(D) Gene set enrichment analysis (GSEA) showing five significantly induced pathways related to the genes upregulated in response to CISAL knockdown in both TSCC cell lines under cisplatin treatment.

(E and F) The inhibitory effect of CISAL knockdown on mitochondrial fission, analyzed by staining with MitoTracker red (E), and apoptosis using flow cytometry (F), after BRCA1 silencing.

***p<0.001, 2-tailed Student's t test. Data are represented as mean ±SEM.