Figure 2.

Analysis of salt tolerance in OsASR1-overexpressing transgenic plants. (A) Four-week-old seedlings (n = 50) after treatment with 200 mM NaCl for 7 d followed by recovery for 5 d. (B) Survival rates of transgenic and WT plants after recovery for 5 d. (C) Na⁺ contents and (D) Na⁺/K⁺ ratio of transgenic and WT plants. (E) Relative water content (RWC) of transgenic and WT plants after NaCl treatment for 7 d. (F) MDA levels, (G) proline contents, and (H) total soluble sugar contents in transgenic and WT plants. Analyses of Na⁺, Na⁺/K⁺ ratio, MDA, proline, and total sugar were carried out with leaves of rice plants treated with 200 mM NaCl for 3 d. Asterisks indicate significant differences between treatments determined by Student's t-test (P < 0.05).