FIGURE 7.

Inhibition of SIAH1 increases expression of NF-κB- and interferon-induced genes. (A–D) HeLa cells were transfected with the indicated siRNA or miRNA mimic and infected with DENV2 (MOI = 5 ffu/ml) 48 h post-transfection. Total RNA was collected in Trizol 48 h p.i., and relative transcripts levels determined by qPCR normalized to β-actin and uninfected control mRNA levels. (E) 48 h after transfection, cells were treated with imiquimod (10 μg/ml) for 24 h. Total RNA was collected and relative mRNA levels determined as described above. (* p-value < 0.05, ** p-value < 0.01, *** p-value < 0.005, **** p-value < 0.001).