Figure 9. . Relative glutamate–cystine antiporter activity assayed by extracellular D4-cystine uptake, determined by steady state levels of intracellular D2-cysteine in cisplatin-resistant ovarian cancer cell lines silenced for cystathionine β-synthase and treated with H₂S for 24 h.

Silencing CBS expression decreases D4-cystine uptake, which is mitigated by the donation of extracellular H₂S. Data representative of at least n = 3 individual experiments.

*p< 0.05.

CBS: Cystathionine β-synthase; H₂S: Hydrogen sulfide.