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. 2020 Feb 10;133(3):jcs235044. doi: 10.1242/jcs.235044

Fig. 2.

Fig. 2.

C-terminal tail truncations uncover unique regulatory sequences. (A) C-terminal sequence alignments of human, mouse, chicken and zebrafish GDE2, and a diagram of the truncation mutants in human GDE2 used in this paper. Note poor sequence conservation in the very C-terminal residues. (B) Subcellular localization of GDE2–HA and its truncation mutants in N1E-115 cells. (C–E) Confocal images of N1E-115 cells co-expressing the indicated GDE2 C-terminal truncations, and Rab5–mCh (C), Rab4–GFP (D) and Rab11–mCh (E). (F) Quantification of GDE2–Rab colocalization (percentage of yellow versus red pixels for ≥25 cells from three independent experiments). Data represent the median±interquartile range of colocalization. ****P<0.0001; ns, not significant (one-way ANOVA). Scale bars: 10 μm.