Fig. 3.

C-terminal truncations impair internalization and recycling of GDE2. (A) Internalization and recycling of GDE2-FL and its C-terminal truncations was followed during 30 min. SH-SY5Y cells inducibly expressing GDE2–HA (Fig. S4A) were surface-labeled with NHS-S-S-Biotin. Labeled GDE2 was allowed to internalize or recycle in the presence of 10% FBS as indicated. Representative western blots are shown. (B) Quantification of band density from three independent experiments. Internalization was normalized to surface GDE2, and recycling to the internalized pool after 30 min. Data represent the mean±s.e.m.