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. 2020 Feb 3;147(3):dev183251. doi: 10.1242/dev.183251

Fig. 3.

Fig. 3.

Undifferentiated SG clusters during the SSC establishment period. (A) UMAP plot of SG from P7 mouse testis. Number of cells in each cluster: SSC-s1 (271), SSC-s2 (328), SSC-2 (479) and Diff-SG (418). (B) UMAP plots of DEGs in the indicated SG cell clusters. (C,D) Pseudotime trajectory analysis of the indicated cell clusters. (E) Heatmap of DEGs in different SG subsets, following the trajectory timeline shown in D. Top: pseudotime direction; left: the gene groups (and the number of genes in each); right: representative enriched genes and the most statistically significant GO terms for each cluster. (F) The expression pattern of marker genes along the pseudotime axis. (G) IF analysis of P7 testes sections from Id4-GFP mice stained with the LHX1 antibody. Scale bar: 20 µm. Right: quantification of double-stained cells. n, number of cells counted. (H) FACS plot of P7 mouse Id4-eGFP testicular cells stained with CD82 antibody. The percentage of positive cells are indicated. Left: only stained with secondary antibody (Ctrl).