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. 2020 Jan 18;9(2):e013784. doi: 10.1161/JAHA.119.013784

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© 2020 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Establishment of a 3D Col‐Tgel biological environment for adipose‐derived mesenchymal stem cells. A, Structure schematic diagrams of Col‐Tgel. B, Glutamine/Lysine enzymatic crosslinking reaction during gelling. C, Scanning electron microscope image of Col‐Tgel. D and E, Scanning electron microscope images of a Col‐Tgel encapsuled adipose‐derived mesenchymal stem cells (ADSC) 3 days after encapsulation. F, 3D view of Col‐Tgel encapsuled ADSCs. Col‐Tgel indicates transglutaminase cross‐linked gelatin; Gln, glutamine; Lys, lysine; PI, propidium iodide; SEM, scanning electron microscope.