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. 2020 Jan 21;9(2):e014276. doi: 10.1161/JAHA.119.014276

Figure 3.

Figure 3

Slug is an unstable protein in human vascular smooth muscle cells (aortic smooth muscle cells [AoSMCs]), whose level is increased by platelet‐derived growth factor‐BB (PDGF‐BB). A, Left panel: Western blot representative of 4 experiments showing the time‐dependent effect of PDGF‐BB (10 ng/mL) on Slug expression. GAPDH is a loading control. A, Right panel: quantification of bands intensity. B, Slug transcripts expression relative to the length of time of a 10 ng/mL PDGF‐BB treatment. Data represent mean±SEM of 4 independent experiments. Results are expressed as fold over control and are normalized relative to hypoxanthine‐guanine phosphoribosyltransferase (HPRT). C, Left panel: Western blot representative of 4 experiments showing the time‐dependent effect of 10 μmol/L MG132 on Slug level (upper) and the effect of 4‐hour MG132 (10 μmol/L) on 2‐hour PDGF‐BB (10 ng/mL)–treated cells (lower); Right panel: quantification of the Western blot bands intensity. For quantification of bands intensity, results (A and C right panels) are normalized relative to GAPDH and expressed as the mean fold over untreated AoSMCs obtained from 4 independent experiments *P<0.05.