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. 2020 Jan 21;9(2):e014276. doi: 10.1161/JAHA.119.014276

Figure 4.

Figure 4

Platelet‐derived growth factor‐BB (PDGF‐BB) stabilizes Slug in the nuclei of rat vascular smooth muscle cells (VSMCs). A through D, Slug protein expression in rat VSMCs infected with an adenovirus delivering hemagglutinin‐human Slug (HA‐Slug) detected by an anti‐hemagglutinin. GAPDH is a loading control. All images are representative of 4 independent experiments. A, Time‐ (upper) and dose‐dependent (lower) effect of PDGF‐BB. The concentration of PDGF‐BB used for the kinetic (upper) is 10 ng/mL. The length of time of the treatments performed with increasing concentrations of PDGF‐BB is 1 hour. B, Time‐dependent effect of 10 μmol/L of MG132 on Slug‐hemagglutinin expression in infected rat VSMCs. C, Snapshots of Slug immunostaining on paraformaldehyde‐fixed 4′,6′‐diamidino‐2‐phenylindole (DAPI)–stained VSMCs treated with vehicle or PDGF‐BB (10 ng/mL, 1 hour). Immunolabelings were analyzed by fluorescence microscopy (×40). Images shown are representative of 4 independent experiments. D, Slug, GAPDH, and histone deacetylase 1 (HDAC1) expression in cytosolic and nuclear fractions from cells treated with vehicle or PDGF‐BB (10 ng/mL, 1 hour). Densitometry analyses of Western blots are given in Figure S3.