Figure 6.

ASA inhibits MLC phosphorylation in platelets stimulated with collagen but not thrombin. Washed human platelets (1000×10⁶/mL) were pretreated or not with ASA (30 μmol/L) for 10 minutes, then stimulated with sCD40L (1000 ng/mL). Aggregation was triggered with suboptimal doses of thrombin (0.025 U/mL) or collagen (0.25 μg/mL). Platelet lysates were resolved in 15% SDS–PAGE and assessed for p‐MLC. The MLC blot is from stripped membranes of the p‐MLC blot. Histograms represent the median of blot data, expressed in optical density (n=4, median±IQR). *P<0.01 vs. control (Kruskal–Wallis followed by Dunn's post hoc test). ASA indicates acetylsalicylic acid; IQR, interquartile range; MLC, myosin light chain; sCD40L, soluble CD40L.