Figure 7.

Effect of ML7 on the potentiating action of sCD40L on platelet aggregation. Washed human platelets (250×10⁶ platelets/mL) were left untreated or pretreated with ML7 (50 μmol/L) for 5 minutes at 37°C. They were preincubated with sCD40L or not (1000 ng/mL) for 30 minutes. Platelet aggregation was induced with suboptimal doses of collagen (0.25 μg/mL, A) or thrombin (0.025 U/mL, B). Histograms represent medians of aggregations (n=9, median±IQR). *P<0.01 vs. other treatments (Kruskal–Wallis followed by Dunn's post hoc test). IQR indicates interquartile range; ML7, selective inhibitor of myosin light chain kinase; sCD40L, soluble CD40L.