Figure 8.
ML7 has no effect on sCD40L signaling. Washed human platelets (1000×106/mL) were pretreated or not with ML7 (50 μmol/L) for 5 minutes, then left untreated or stimulated with sCD40L (1000 ng/mL) for 5 minutes. Platelet lysates were resolved in 10% SDS–PAGE and assessed for p‐TAK1 and stripped for p‐IκBα, p‐p38, and actin. Histograms represent the median of blot data, expressed in optical density (n=5, median±IQR). *P<0.01 vs. control (Kruskal–Wallis followed by Dunn's post hoc test). IQR indicates interquartile range; ML7, selective inhibitor of myosin light chain kinase; sCD40L, soluble CD40L; TAK1, transforming growth factor‐β–activated kinase 1.