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. 2020 Feb 14;11:183. doi: 10.3389/fimmu.2020.00183

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Copyright © 2020 Jones, Brown, Phythian-Adams, Ivens, Cook and MacDonald.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The impact of Mbd2 deficiency on mRNA expression by colon epithelial cells during colitis. (A) Volcano plot depicts differential gene expression profile of mRNA as determined by microarray of colon epithelial cells (CD45⁻ Lin⁻ F4/80⁻ CD3⁻ EpCAM⁺) isolated by flow cytometry after 6 consecutive days DSS treatment in Mbd2^−/− or WT littermate co-housed mice. (B) Heat map of relative gene expression values from selected significant (log₂ fold change ± 1, P < 0.05) loci and selected up (C) and down (D) (P < 0.05) pathways from GOterm analysis from (A) (log₂ normalized intensity, one-fold change-filtered, P < 0.05), 5 biological replicates per genotype.