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. 2020 Feb 3;5(6):2893–2898. doi: 10.1021/acsomega.9b03739

Figure 1.

Figure 1

MTCap-CBMCef-sfGFP expression in E. coli and immobilized to microcrystalline cellulose. (A) Schematic representation of the fusion protein. MTCap, MT from C. pagurus (GenBank accession no. AM743087.1); CBMCef, family 2 CBM from the endoglucanase A of C. fimi (GenBank accession no. M15823.1); sfGFP, superfolder GFP. Two linkers L1 (GSGGAGGS) and L2 (GGGSPTGG) were used to connect the three modules. (B) Cell precipitate of an uninduced transformant harboring pET-MTCap-CBMCef-sfGFP (1) and IPTG-induced transformant harboring pET-MTCap-CBMCef-sfGFP (2) “Photograph courtesy of Guozeng Wang, Copyright 2019”. (C) Microcrystalline cellulose (1) and MTCap-CBMCef-sfGFP@cellulose complexes (2) “Photograph courtesy of Qing Xiao, Copyright 2019”.