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. 2020 Feb 14;9:1576. doi: 10.3389/fonc.2019.01576

Figure 4.

Figure 4

Senescent cells are eliminated by DQ treatment. (A) Young/senescent HOK and skin fibroblasts were treated with DMSO or DQ for 24 h, and collected for apoptosis analysis using flow cytometry (n = 3), repeated three times independently (mean with SD. n = 3, ***P < 0.001; independent samples Student's t-test; ns, no significance). (B) HOK and skin fibroblasts were co-stained with calcein-AM (Invitrogen)/PI to visualize live cells (green fluorescence) and dead or late apoptotic cells (red fluorescence) (n = 3; scale bar, 100 μm). (C) Apoptosis markers PARP, caspase3, and cleaved caspase3 expression levels in young/senescent HOK and skin fibroblasts after being incubated with DMSO or DQ for 24 h. Three independent experiments started with cell plating.